Role of Killer cell immunoglobulin-like receptors (KIR) genes in stages of HIV-1 infection among patients from Burkina Faso.

Sorgho PA, Djigma FW, Martinson JJ, Yonli AT, Nagalo BM, Compaore TR, Diarra B, Sombie HK, Simpore A, Zongo AW, Ouattara AK, Soubeiga STR, Traore L, Yelemkoure ET, Kiendrebeogo IT, Roberts LR, Simpore J. ; Biomol Concepts. 2019 Dec 19 ;10(1):226-236. doi : 10.1515/bmc-2019-0024. PMID : 31863692
https://www.ncbi.nlm.nih.gov/pubmed/31863692

Abstract

Objectives : A cluster of specialized KIR genes of specialized KIR genes has been shown to be associated with susceptibility or resistance to viral infections in humans. Therefore, this pilot study, this pilot investigation sought to determine the frequencies of KIR genes human immunodeficiency virus type 1( HIV-1) patients and establish their potential clinical involvement in disease progression and staging.
Methods :
HIV-1 infected and healthy individuals were selected for this study. Hepatitis B surface antigen (HBsAg), anti-HCV antibodies and anti-HIV-1/2 antibody/ antigen were screened using a 4th generation ELISA assay (Cobas e 411 Analyzer, Roche Diagnostics GmbH Mannheim, Germany). SSP-PCR was used to evaluate the frequencies of KIR genes. CD4+ T counts and HIV-1 viral load were measured in patients using respectively BD FACSCount and Abbott m2000rt instruments.
Results : We found a significant association between the frequencies of KIR2DL2 (OR=4.41 ; p < 0.001), KIR2DS2 (OR=4.76 ; p < 0.001), KIR2DS3 (OR=2.27 ; p=0.004), KIR2DS4 (OR=1.76 ; p=0.026), KIR3DS1 (OR=2.43 ; p=0.016) and HIV-1 infection ; whilst the KIR3DL1 gene (OR= 0.39 ; p < 0.001) was associated with protection against HIV-1 infection. HIV-1 replication was found to be associated with the presence of KIR2DS2 (OR=6.08, p = 0.024). In contrary the pseudogene KIR2DP1 (OR=0.39 ; p=0.026) were linked to a protective status with the highest number of lymphocyte T CD4 counts.
Conclusion : Our data showed that KIR2DL2, KIR2DS2, KIR2DS3, KIR2DS4, and KIR3DS1 were significantly associated with HIV-1 infection whereas KIR3DL1 was associated with protection against HIV-1 infection. Further investigations are needed to fully comprehend the clinical significance of KIR genes in HIV disease progression.

KEYWORDS :Burkina Faso ; HIV-1 ; KIR gene ; T CD4 ; Viral Load



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