First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso


Tiemtoré Rahimatou Yasmine, Mètuor Dabiré Amana, Zohoncon Theodora Mahoukèdè, Bangré Aminata Yasmine, Sougue Serge, Zongo Jacob, Simpore Jacques. International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 4, No. 1, 2019, pp. 7-12. doi : 10.11648/j.ijbbmb.20180401.12

http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=246&doi=10.11648/j.ijbbmb.20180401.12

Abstract
Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics : Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.

Keywords : Broad-spectrum Beta-lactamases (ESBL), PER Gene, PCR



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