New astrovirus in human feces from Burkina Faso.

Phan TG, Nordgren J, Ouermi D, Simpore J, Nitiema LW, Deng X, Delwart E.

J Clin Virol. 2014 Jun ;60(2):161-4. doi : 10.1016/j.jcv.2014.03.024. Epub 2014 Apr 13.

PMID : 24775733 [PubMed - in process] ; ;

BACKGROUND : A significant fraction of cases of diarrhea, a leading cause of childhood mortality worldwide, remain unexplained.
OBJECTIVES : To identify viruses in unexplained cases of diarrhea using an unbiased metagenomics approach.
STUDY DESIGN : Viral nucleic acids were enriched from the feces from 48 cases of unexplained diarrhea from Burkina Faso, sequenced, and compared against all known viral genomes.
RESULTS : The full genome of a highly divergent astrovirus was sequenced in a sample co-infected with parechovirus 1. RT-PCR identified a single astrovirus infection in these 48 patients indicating a low prevalence. Human astrovirus-BF34 was most closely related to mamastrovirus species 8 and 9 also found in human with which it shared 62%, 74%, and 57% amino acid identities over its protease, RNA dependent RNA polymerase and capsid proteins, respectively.
CONCLUSIONS : Burkina Faso astrovirus is proposed as prototype for a novel species in the genus Mamastrovirus, here tentatively called Mamastrovirus 20, representing the fifth human astrovirus species.

KEYWORDS : Astrovirus ; Children ; Diarrhea ; Metagenomics ; Species

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