β-lactamase Molecular Classes Linked to Production Ways from Clinical Isolates in Burkina Faso/West Africa.

Boukare Zeba, Jacques Simpore and Odile Germaine Nacoulma ;
2007 | Volume : 7 | Issue : 1 | Page No. : 98-102
DOI : 10.3923/jas.2007.98.102

http://scialert.net/abstract/?doi=jas.2007.98.102

Abstract

β-lactamase production ways and inhibition patterns were investigated in cell suspensions of clinical isolates. The purpose of this research was prior to investigate the different β-lactamase molecular classes occurring in Burkina Faso owing to the local practice of β-lactam antibiotics. The use of specific inhibitors enabled to draw up an inhibition profile and consequently to assign an enzyme to accurate molecular class of β-lactamase. At the same time, β-lactamase expression ways were explored and correlated to the inhibition profiles. The current results pointed out two main groups of organisms. The first group of isolates secretes β-lactamases mainly by inducible way. Interestingly this bacterial group carries molecular class C of β-lactamase. The second group of isolates uses partial inducible and partial constitutive way for enzyme expression. These bacteria mostly carry molecular class A of β-lactamases. The sole exception encountered during these investigations is an organism, expressing exclusively by the constitutive way an enzyme that is found to belong to molecular class B of β-lactamases.



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